《植物生理学报》 2009, 45(11): 1093-1097

一个新的茶树黄酮醇合酶基因的克隆和表达分析

谢素霞, 张媛, 郭凯琳, 王磊, 袁红雨*

信阳师范学院生命科学学院, 河南信阳464000

通信作者：袁红雨；E-mail: hongyu_yuan@263.net；Tel: 0376-6392267

摘　要：

Cs-COR113 (GenBank 登录号: FE942098)为一受冷诱导的茶树黄酮醇合酶基因的 cDNA 片段, 采用 RACE 技术克隆 了这一基因的全长 cDNA, 命名为 CsFLS (GenBank 登录号: FJ577509)。CsFLS cDNA 序列全长为 1303 bp, 5'-UTR 和 3'- UTR 分别长 91 bp 和 175 bp, 包含一个编码 336 个氨基酸的完整开放阅读框。序列分析显示, CsFLS 与烟草、矮牵牛、欧 芹、拟南芥的黄酮醇合酶的同源性分别为 74%、75%、75% 和63%, 含有 2- 酮戊二酸依赖性双加氧酶家族 2 个保守的基序, 以及与黄酮醇合酶正确折叠有关的 2 个保守的甘氨酸残基。CsFLS 的表达受低温诱导, 但不受 ABA 诱导。

关键词：茶树; 黄酮醇合酶; 基因表达

收稿：2009-08-20   修定：2009-10-14

资助：国家自然科学基金(30570137)和河南省高等学校新世纪优秀人才支持计划 (2005HANCET-14)。

Cloning and Expression Analysis of a Flavonol Synthase Gene from Camellia sinensis

XIE Su-Xia, ZHANG Yuan, GUO Kai-Lin, WANG Lei, YUAN Hong-Yu*

Faculty of Life Sciences, Xinyang Normal College, Xinyang, Henan 464000, China

Corresponding author: YUAN Hong-Yu; E-mail: hongyu_yuan@263.net; Tel: 0376-6392267

Abstract:

Cs-COR113 is the cDNA fragment of a cold-induced flavonol synthase (FLS) gene of tea (Camellia sinensis L.). The full-length cDNA, which was cloned using RACE (rapid amplification of cDNA ends) strategy (designated as CsFLS, GenBank accession number: FJ577509), was 1 303 bp in length with a 91-bp 5'-UTR and a 175-bp 3'-UTR, containing an open reading frame of 336 amino acids. CsFLS protein had homology to other flavonol synthase from Nicotiana tabacum, Petunia hybrida, Petroselinum crispum, and Arabidopsis thaliana, showing 74%, 75%, 75%, and 63% in amino acid identity, respectively. CsFLS contained two conserved motifs of the 2-oxoglutarate-dependent dioxygenases and two glycine residues required for proper folding of the FLS polypeptide. The expression of CsFLS was induced by low temperature, but not by ABA.

Key words: Camellia sinensis L.; flavonol synthase; gene expression